Plant Transcription Factor Database
Previous version: v3.0
Capsella rubella
GeBP Family
Species TF ID Description
Carubv10001022mGeBP family protein
Carubv10002727mGeBP family protein
Carubv10004944mGeBP family protein
Carubv10005097mGeBP family protein
Carubv10005452mGeBP family protein
Carubv10006604mGeBP family protein
Carubv10007629mGeBP family protein
Carubv10011873mGeBP family protein
Carubv10012756mGeBP family protein
Carubv10013683mGeBP family protein
Carubv10013903mGeBP family protein
Carubv10015397mGeBP family protein
Carubv10015438mGeBP family protein
Carubv10016166mGeBP family protein
Carubv10016201mGeBP family protein
Carubv10020457mGeBP family protein
Carubv10023535mGeBP family protein
Carubv10025028mGeBP family protein
Carubv10025071mGeBP family protein
GeBP Family Introduction

Understanding the role of transcription factors (TFs) is essential in reconstructing developmental regulatory networks. The plant-specific GeBP TF family of Arabidopsis thaliana (Arabidopsis) comprises 21 members, all of unknown function. A subset of four members, the founding member GeBP and GeBP-like proteins (GPL) 1, 2, and 3, shares a conserved C-terminal domain. Here we report that GeBP/GPL genes represent a newly defined class of leucine-zipper (Leu-zipper) TFs and that they play a redundant role in cytokinin hormone pathway regulation. Specifically, we demonstrate using yeast, in vitro, and split-yellow fluorescent protein in planta assays that GeBP/GPL proteins form homo- and heterodimers through a noncanonical Leu-zipper motif located in the C-terminal domain. A triple loss-of-function mutant of the three most closely related genes gebp gpl1 gpl2 shows a reduced sensitivity to exogenous cytokinins in a subset of cytokinin responses such as senescence and growth, whereas root inhibition is not affected. We find that transcript levels of type-A cytokinin response genes, which are involved in the negative feedback regulation of cytokinin signaling, are higher in the triple mutant. Using a GPL version that acts as a constitutive transcriptional activator, we show that the regulation of Arabidopsis response regulators (ARRs) is mediated by at least one additional, as yet unknown, repressor acting genetically downstream in the GeBP/GPL pathway. Our results indicate that GeBP/GPL genes encode a new class of unconventional Leu-zipper TF proteins and suggest that their role in the cytokinin pathway is to antagonize the negative feedback regulation on ARR genes to trigger the cytokinin response.

Chevalier F, Perazza D, Laporte F, Le Henanff G, Hornitschek P, Bonneville JM, Herzog M, Vachon G
GeBP and GeBP-Like Proteins Are Noncanonical Leucine-Zipper Transcription Factors That Regulate Cytokinin Response in Arabidopsis
Plant Physiol. 2008 Mar;146(3):1142-54.
PMID: 18162594